翻訳と辞書 Words near each other ・ Transform Scotland ・ Transform theory ・ Transform, clipping, and lighting ・ Transformation ・ Transformation (Don Preston album) ・ Transformation (function) ・ Transformation (genetics) ・ Transformation (journal) ・ Transformation (law) ・ Transformation (music) ・ Transformation (Signal Aout 42 album) ・ Transformation (Tal Wilkenfeld album) ・ Transformation (warfare) ・ Transformation between distributions in time–frequency analysis ・ Transformation design ・ Transformation efficiency ・ Transformation geometry ・ Transformation in economics ・ Transformation language ・ Transformation mask ・ Transformation matrix ・ Transformation Ministries ・ Transformation obsession ・ Transformation of culture ・ Transformation of text ・ Transformation of the United States Army ・ Transformation optics ・ Transformation playing card ・ Transformation Priority Premise ・ Transformation problem Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Transformation efficiency ： ウィキペディア英語版 Transformation efficiency Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure. Transformants are cells that have taken up DNA (foreign, artificial or modified) and which can express genes on the introduced DNA. == Measure of transformation efficiency == Transformation effiency should be determined under conditions of cell excess. The number of viable cells in a preparation for a transformation reaction may range from 2x108 to 1011; most common methods of ''E. coli'' preparation yield around 1010 viable cells per reaction. The standard plasmids used for determination of transformation efficiency in ''Escherichia coli'' are pBR322 or the smaller pUC series of vectors, different vectors however may be used to determine their transformation efficiency. 10-100 pg of DNA may be used for transformation, more DNA may be necessary for low-efficiency transformation (generally saturation level is reached at over 10 ng).〔(【引用サイトリンク】title= Calculating Transformation Efficiency )〕 After transformation, 1% and 10% of the cells are plated separately, the cells may be diluted in media as necessary for ease of plating. Further dilution may be used for high efficiency transformation. Transformation efficiency is measured in transformants or colony forming unit (cfu) per μg DNA used. A transformation efficiency of 1x108 cfu/μg for a small plasmid like pUC19 is roughly equivalent to 1 in 2000 molecules of the plasmid used being transformed. In ''E. coli'', the theoretical limit of transformation efficiency for most commonly used plasmids would be over 1x1011 cfu/μg. In practice the best achievable result may be around 2-4x1010 cfu/μg for a small plasmid like pUC19, and considerably lower for large plasmids. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Transformation efficiency」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.